amphibians, which include Xenopus laevis (X. laevis) and Xenopus tropicalis (X. tropicalis), are one such group of animals with high regenerative capacity (Dinsmore, 1991). Xenopus tropicalis, on the other hand, possesses a diploid genome and a short generation time, ... (C and D) in stage 55 Xenopus laevis and Xenopus tropicalis tadpoles. The rapid growth of the National Xenopus Resource has required effective implementation and optimization of these protocols. Genetic Map of Xenopus tropicalis Jamie Davies The kidney development database. The cardiac versus skeletal expression patterns of actins are probably sequence-dependent. tropicalis shares advantages of X. laevis as a classic embryologic system, but its simpler genome and shorter generation time make it more convenient for multigenerational genetic, genomic, and transgenic … Xenopus tropicalis Taxonomy ID: 8364 (for references in articles please use NCBI:txid8364) current name. Life cycle, evolutionary position and regenerative potential of Xenopus. Xenopus tropicalis and Xenopus laevis environmental parameter standard operating procedure (Kroll lab) From Xenbase. Reviewed-Annotation score: -Experimental evidence at transcript level i. Digitized images and developmental data from Nieuwkoop and Faber (1994) Normal Table of Xenopus laevis (Daudin). Xenopus tropicalis (Gray, 1864) basionym: Silurana tropicalis Gray, 1864. homotypic synonym: Xenopus (Silurana) tropicalis. From the early 1700s to the present, Xenopus have been studied as animal … The word Xenopus means "strange foot" and laevis means "smooth". Garland Publishing Inc, New York ISBN 0-8153-1896-0. Pattern and morphogenesis of presumptive superficial mesoderm in two closely related species, Xenopus laevis and Xenopus tropicalis. They must rely on external heat sources. This is the first amphibian genome to be sequenced, enabling new insights into vertebrate evolution and the last common ancestor of tetrapods. Due to the tetraploid nature of Xenopus laevis, each of these three alpha actin genes is present in at least two copies. Xenopus Laevis should be housed at 20-23 degrees C (68-75 degrees F). Exhibits a high amplitude circadian rhythm with maximal levels in early evening (PubMed:12573214). Room temperature (controlled automatically by building … Pedro's BioMolecular Research Tools A Collection of WWW Links to Information and Services Useful to Molecular Biologists. Kalt MR (1976) Morphology and kinetics of spermatogenesis in Xenopus laevis. Ngn1 is expressed in the otic placode at early embryological stages (up to NF22), but with somewhat stronger expression in Xenopus tropicalis than in Xenopus laevis. 1. Session et al. The mesoderm, comprising the tissues that come to lie entirely in the deep layer, … Xenopus Tropicalis should be housed at 23-28 degrees C (75 -80 degrees F). A small, fast‐breeding, diploid relative of the frog Xenopus laevis, Xenopus tropicalis, has recently been adopted for research in developmental genetics and functional genomics.X. Garland Publishing Inc, New York ISBN 0-8153-1896-0. Probable transcription factor. Xenopus tropicalis has been developed as a model organism for developmental biology, ... Three ngn subfamilies (Ngnr1–3) have been isolated in both Xenopus laevis and Xenopus tropicalis (Nieber et al., 2009). Xenopus laevis tropicalis. Phosphatase which catalyzes the conversion of NADP(+) to NAD(+) and of NADPH to NADH (By similarity). Likewise, X. tropicalis has been selected over X. laevis as the subject of a complete genome sequencing project for the same reason. Introduction. *Significantly greater or less (p < 0.05) than the corresponding values for controls. XDB is a Web data catalogue of NIBB/NIG Xenopus laevis EST project. Xenopus commonly known as the clawed frog) is a genus of aquatic frogs native to sub-Saharan Africa.. Twenty species are known. Xenopus laevis is a scavenger and eats living, dead, or dying arthropods and other pieces of organic waste. Available as frozen sperm, please allow 8 months for live animals. 26. However, X. laevis has an allotetraploid genome precluding its use in forward genetic analysis. Robust and efficient protocols for fertilization and early embryo care of Xenopus laevis and Xenopus tropicalis are essential for experimental success, as well as maintenance and propagation of precious animal stocks. … It has a voracious appetite and attacks anything that passes in front of it. Xenopus laevis has long been a popular model for studies of development and, ... and genetically much more convenient diploid Xenopus tropicalis (Vogel, 1999). Like Xenopus laevis, X. tropicalis was formerly used in pregnancy tests (Bergerard & Lamotte 1949). Introduction. Embryos develop externally and tadpoles are transparent, features that facilitate experimental manipulation and post-factum analysis of animals (Robert and Ohta, 2009; Banach and Robert, 2017). The African clawed frog (Xenopus laevis, also known as the xenopus, African clawed toad, African claw-toed frog or the platanna) is a species of African aquatic frog of the family Pipidae.Its name is derived from the three short claws on each hind foot, which it uses to tear apart its food. X. laevis is the species of frog most often used to generate egg extracts. Verlag der Zeitschrift für Naurforschung, Germany Google Scholar. Description: (Xic-GFP) 4.7 kb X. tropicalis p27 Xic1 promoter driving GFP expression in the neural tube and the eye. Experiments were repeated three times. 2010). The frog Xenopus laevis has provided significant insights into developmental and cellular processes. Xenopi cannot raise their body temperature by producing metabolic heat. Because X. tropicalis is diploid and X. laevis is tetraploid, important inferences can be made about genome evolution. Silurana tropicalis Gray, 1864. XenOrtho DB (Xenopus Ortholog DataBase) is a database in which ortholog genes of Xenopus tropicalis, Xenopus laevis and humans are listed. Amphibian Research Center) through collaboration between the Xenopus Community in Japan (XCIJ) and National Bio-Resource Project (NBRP). Lateral line systems, usually found in fish, detect vibrations in the water. Xenopus tropicalis Taxonomy ID: 8364 (for references in articles please use NCBI:txid8364) current name. al. Xenopus laevis (African clawed frog) Status. Xenopus laevis (XLA) is an allotetraploid species which appears to have undergone whole-genome duplication after the interspecific hybridization of 2 diploid species closely related to Silurana/Xenopus tropicalis (XTR). Component of the circadian clock or downstream effector of clock function (PubMed:12573214). A transgenic line of X. tropicalis was used to examine the process of cornea-lens transdifferentiation in which green fluorescent protein (GFP) is expressed in differentiated lens cells under the control of the Xenopus gamma1-crystallin promoter element. However, another species of frog Xenopus tropicalis can also be a source. It was produced by Dr. Fukui (Faculty of Science and Engineering, Chuo Univ.) Xenopus laevis and Xenopus tropicalis have long been used to drive discovery in developmental, cell, and molecular biology. Jump to: navigation, search. Xenopus tropicalis (Gray, 1864) homotypic synonym: Xenopus (Silurana) tropicalis. Carruthersa, S., et. These dual frog species boast experimental strengths for embryology including large egg sizes that develop externally, well‐defined fate maps, and cell‐intrinsic sources of nutrients that allow explanted tissues to grow in culture. Function i. The early development of Xenopus Laevis, an atlas of the histology. Download Xenopus Genomes; X. laevis 9.2 (J-Strain) on JBrowse; X. laevis 9.2 (J-Strain) on UCSC; X. laevis 1.0 (wildtype) on JBrowse; X. tropicalis 10.0 (Nigerian) on JBrowse; X. tropicalis 9.1 (Nigerian) on JBrowse; X. tropicalis 9.1 (Nigerian) on UCSC; Xenbase UCSC Track Hub; Veenstra Lab UCSC Track Hub; Other amphibian genomes on JBrowse; Expression. Procedures input by VGP link to Kroll lab Xenopus environmental procedure SOP document at Washington University animal facility: Modified format below: A. Xenopus egg extract is a lysate that is prepared by crushing the eggs of the African clawed frog Xenopus laevis.It offers a powerful cell-free (or in vitro) system for studying various cell biological processes, including cell cycle progression, nuclear transport, DNA replication and chromosome segregation.It is also called Xenopus egg cell-free system or Xenopus egg cell-free extract. It is found in Nigeria, and from Sudan to South Africa where they are plentiful in the rivers and ponds within the southeastern portion of Sub Saharan Africa. Xenopus laevis and Xenopus tropicalis have been used extensively in developmental and cell biology, biochemistry, functional genomics and immunology. Shook DR(1), Majer C, Keller R. Author information: (1)Department of Biology, University of Virginia, Gilmer Hall, Charlottesville, VA 22903, USA. If the water temperature is sub optimal... frogs will not eat and their metabolism and immune systems will be depressed. and Dr. Igawa (Hiroshima Univ. The African Clawed Frog (Xenopus laevis) is also known to tropical fish keeping enthusiasts as the Xenopus, African Clawed Toad, or African Claw Toed frog and is the only amphibian that actually has claws. Xenopus laevis (also called the African Clawed frog due to the sharp, black claws in the hind inner three toes) is an aquatic amphibian of the Order Anuran and the family Pipidae.Xenopus are a long-lived, aquatic amphibian commonly used in teaching classrooms and in research laboratories. In the present study, membrane integrity and DNA stability of Xenopus laevis and Xenopus tropicalis spermatozoa were evaluated in response to cryopreservation with or without activation, a process that happens upon exposure to water to spermatozoa of some aquatic species. Shows a small preference for NADPH over NADP(+) (By similarity). It uses extremely sensitive fingers, an acute sense of smell, and its lateral line systems to locate food. Axeldb A database focussing on gene expression in the frog Xenopus laevis. Reference: EXRC. Xenopus tropicalis was somewhat less sensitive to 6‐AN, semicarbizide and atrazine when tested at 27 °C than at 23 °C. We have demonstrated here that monospermy in the clawed frog, Xenopus tropicalis, as well as in X. laevis, is ensured by a fast, electrical block to polyspermy on the egg plasma membrane after the entry of the first sperm, which is mediated by the positive-going fertilization potential. Xenopus contains at least three sarcomeric alpha actin genes that are preferentially expressed in either heart or skeletal muscle.

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